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. 2022 Feb 24;13:829566. doi: 10.3389/fpls.2022.829566

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Copyright © 2022 Nguyen, Hayes, Harris and Sutton.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Absolute quantitative real-time PCR (qRT-PCR) analysis of expression of chickpea GIF1, GIF1L, GRF4, GRF5L, and Ca4_TIFY4B in NILs carrying contrasting Ca4_TIFY4B alleles, Rupali (R) or Genesis836 (G). NILs were derived from RIL50, heterozygous across the vigor region Ca4_Vqtl. Gene expression was analyzed for whole shoot and root harvested at 9 days after sowing, youngest fully emerged leaves sampled at 7 weeks after sowing, and developing seed. Means ± SD are shown for n = 3 or 4. There were no significant differences in expression for any of the tested genes in these tissues (P > 0.05).