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. 2022 Feb 7;298(3):101689. doi: 10.1016/j.jbc.2022.101689

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

DASPMI dye DSF derivative fluorescence data for 2.5 μM h2E2 in PBS buffer: control, AAPH-treated, and AAPH/Met-treated (24 h at 40 °C) mAb as a function of cocaine ligand added (0–1000 μM).Top panel, displays control mAb data, middle panel is data obtained using the AAPH/Met-mAb, whereas the bottom panel is AAPH-treated mAb data (both treated for 24 h at 40 °C). All samples were analyzed in duplicate, using the concentrations of cocaine specified by the legend and the trace colors in the figure. The concentrations of ligands (0–1000 μM) for each data trace are specified by the legend embedded in the figure. AAPH, 2,2′-azobis(2-amidinopropane) dihydrochloride; DASPMI, 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide; DSF, differential scanning fluorimetry; mAb, monoclonal antibody.