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. 2022 Feb 7;298(3):101689. doi: 10.1016/j.jbc.2022.101689

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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DASPMI dye DSF derivative fluorescence data for 2.5 μM h2E2 in PBS buffer: 5 mM AAPH-treated and AAPH/Met-treated (24 h at 40 °C) mAb as a function of ligand added (0–1000 μM) for cocaineand two mAb high-affinity cocaine metabolites, cocaethylene (CE) and benzoylecgonine (BE).A, top to bottom, CE, cocaine, and BE ligand titrations for the AAPH-treated mAb. B, top to bottom, CE, cocaine, and BE ligand titrations for the AAPH/Met-treated mAb. The concentrations of ligands (0–1000 μM) for each data trace are specified by the legend embedded in the figure. AAPH, 2,2′-azobis(2-amidinopropane) dihydrochloride; DASPMI, 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide; DSF, differential scanning fluorimetry; mAb, monoclonal antibody.