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. 2022 Feb 7;298(3):101689. doi: 10.1016/j.jbc.2022.101689

Figure 6.

Figure 6

DASPMI DSF derivative peak temperature maximums (TmDs) for control and 24 h, 40 °C 5 mM AAPH/260 mM Met-treated 2.5 μM h2E2 mAb as a function of ligand concentration.Filled symbols are control mAb data, whereas open symbols are the AAPH/Met mAb-oxidized samples, titrated with 0 to 1000 μM CE, cocaine, or BE ligands. TmB values for each sample are all close to TmD values at each ligand concentration, and thus TmB values are not shown to increase the visual clarity of presentation. AAPH, 2,2′-azobis(2-amidinopropane) dihydrochloride; BE, benzoylecgonine; CE, cocaethylene; DASPMI, 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide; DSF, differential scanning fluorimetry.