Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 7;298(3):101689. doi: 10.1016/j.jbc.2022.101689

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Liquid chromatography–mass spectrometry identification of tryptophan residue oxidation in the cocaine-binding site of h2E2 upon oxidation with AAPH.A and B, extracted ion profiles representing the indicated light-chain Trp91 peptide and met87 peptides, respectively, in the oxidized forms after AAPH-only (upper), AAPH + methionine (middle), and no treatment (lower) with 2.5 h thermolysin digestion. Each sample profile is scaled equally to the maximum intensity observed for each of the two peptides, as indicated. C, comparative intensities of the indicated oxidized peptides in A and B, scaled to 100% maximal intensity for each peptide. D, comparative intensities for the heavy-chain Trp33 peptide oxidized at Trp33 alone, Trp33 and met34, and at met248, all scaled to 100% maximal intensity of each peptide. AAPH, 2,2′-azobis(2-amidinopropane) dihydrochloride.