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. 2022 Mar 3;51:102269. doi: 10.1016/j.redox.2022.102269

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© 2022 The Authors. Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — LMH001 specificity and efficacy as p47^phoxinhibitor. a. LMH001 structure. b. Kd and Ki values of LMH001 tested by fluorescence polarization. mP: millipolarization units. c-d. HPLC-MS/MS detection of LMH001 in COS-p22/p47^phox cells with or without PMA stimulation. c. LMH001 detected in p47^phox immunoprecipitation (IP) pellets. d. LMH001 detected in the fractions left after p47^phox IP. Mup47: p47^phox S303-4/379A mutation. Results were expressed as fold changes of Cos-p22^phox cells transfected with an empty vector (Vec) (values = 1). e-h. Lucigenin-chemiluminescence. e. O₂^.- production by xanthine plus xanthine oxidase (X + XO) or PMA-stimulated COS-phox cells. f. O₂^.- production by mouse peripheral blood mononuclear cells (PBMC). g-h. Oxidative burst response of PBMC isolated from mice after 14 d ip injection of LMH001. MLU: mean light units. *P < 0.05 for indicated values versus vehicle control values. n = 6 independent cell cultures or isolations.