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. 2022 Mar 3;51:102269. doi: 10.1016/j.redox.2022.102269

Fig. 2.

Fig. 2

LMH001 inhibition of AngII-induced endothelial cell ROS production. a,b,d: lucigenin-chemiluminescence. a. Dose response of LMH001 inhibition of AngII-induced O2.- production by primary mouse coronary microvascular endothelial cells (CMEC). MLU: mean light units. b. Effects of different inhibitors on AngII-induced O2.- production by WT CMEC. Results were expressed as % of AngII values (100%). c. DHE fluorescence HPLC detection of ROS production by human pulmonary microvascular endothelial cells. 2-HE+ (O2.-), E+ (other ROS). AUC: area under curve, d. O2.- production by WT and p47phox KO CMEC. e. H2O2 production by WT and p47phox KO CMEC detected by amplex red assay. Results were expressed as % of vehicle control values (100%). *P < 0.05 for indicated values versus vehicle control (or control) values. †P < 0.05 for indicated values versus vehicle AngII (or AngII) values. n = 6 independent cell cultures. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)