Fig. 5.

LMH001 inhibition of AngII-induced aorta ROS production, aortic wall thickness and MMP activation. a.In situ DHE fluorescence (red) detection of aorta ROS production. b. H&E staining for aortic aneurysm and morphology. Aorta wall thickness was measured using ImageJ software. c. Left panels: Representative images of in situ zymography detection of matrix metalloproteinase (MMP) activities (green fluorescence). Nuclei were labelled by DAPI (blue) to visualized cells. Right panels: In gel zymography detection of MMP activity. The optical densities of MMP2 bands were quantified, normalized to the loading control (α-tubulin) and expressed as fold changes against vehicle WT control values. *P < 0.05 for indicated versus vehicle control values. †P < 0.05 for indicated values versus vehicle AngII values. n = 9 mice/group. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)