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Melting curves and fluorescence measurements were determined for WN197 and WN170.
| Compound | ΔTm (°C) | Kapp (10^7 M-1) | EtBr displacement |
|---|---|---|---|
| WN197 | 16.6 | 15.005 ± 0.290 | 90% |
| WN170 | 16.1 | 2.436 ± 0.883 | 87% |
Variations in melting temperature (ΔTm=Tm drug−DNA complex−Tm DNA alone) were performed at a ratio of 0.5. Apparent binding constant were measured by fluorescence using [EB]/[DNA] = 1.26. Data were the mean of at least three independent experiments.
