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RNA-Seq results were validated by qPCR. Two randomly selected down-regulated DEG expression (A) from RNA-Seq and three up-regulated DEG (B) expression were detected by qPCR, and (C) four typical genes related to BBB. *p < 0.05, **p < 0.01, and ***p < 0.00. Student’s t-test was used for comparisons.
