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. Author manuscript; available in PMC: 2022 Mar 10.
Published in final edited form as: J Proteome Res. 2020 Jun 8;19(8):2989–2996. doi: 10.1021/acs.jproteome.0c00024

Figure 5.

Figure 5.

Prostate cancer tissues analyzed upon multiple enzymatic digestions. A prostate cancer tissue section undergoing PNGase F treatment (A,D,G), Endo F3 treatment (B,E,H) or sequential PNGase F treatment following a wash of the Endo F3-treated tissue (C,F,I). A known core-fucosylated glycan, A2G2F distribution is shown for the PNGase F mass shift of 1809.6393 m/z (A−C) and for the Endo F3-treated, a mass shift of 1460.5023 m/z (D−F). Finally, high-mannose glycan Man8 (1743.5810 m/z) distribution is shown (G−I). Scale bar and intensity bar are included.