Figure 4.

Propofol postconditioning upregulates AdipoR2 and downregulates miR-200c-3p. (A) Binding sites of miR-200c-3p and AdipoR2 were predicted using TargetScan. (B) Dual-luciferase reporter assay was used to detect the targeting relationship between miR-200c-3p and AdipoR2. (C) AdipoR2 mRNA expression levels in diabetic myocardial ischemic reperfusion rat model following propofol postconditioning were detected by RT-qPCR, n=6. (D) AdipoR2 mRNA expression levels in H9C2 cells following propofol postconditioning were detected by RT-qPCR. Three independent cell tests were performed. Data are presented as the mean ± standard deviation. Unpaired Student's t-test was used for comparisons between two groups in panel B, and one-way ANOVA followed by Tukey's post hoc test was used for comparisons between multiple groups in panels C and D. **P<0.01. AdipoR2, adiponectin receptor 2; miR, microRNA; MI/RI; RT-qPCR, reverse transcription-quantitative PCR; UTR, untranslated region; NC, negative control; P25, 25 µM propofol; Wt, wild-type; Mut, mutant; DS, sham group; DI, MI/RI group; DP, MI/RI + propofol group; HG, high glucose; H/R, hypoxia/reperfusion; rno, Rattus norvegicus.