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. 2022 Feb 24;12:830816. doi: 10.3389/fonc.2022.830816

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Copyright © 2022 Yang, Zou, Li, Liu, Yan, Chen, Zhao, Guo, Huang, Li, Zhu and Chen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Longitudinal radiograph and ctDNA detection of one representative CRC patient. (A) Radiographic features of tumor lesions in a 73-year-old male with colorectal cancer liver metastasis. PR, partial response; SD, stable disease; PD, progressive disease. (B) Comparison of the changes of tumor diameter, CEA and CA199 in patient 1(P1). Cet: Cetuximab; IRI, Irinotecan; Rego, Regorafenib. (C) Example of a serial ctDNA testing in P1 showing emergence of clonal alterations with treatment process. The changes of line thickness indicate the changes of variant allele frequency (VAF) of genomic alterations. The thicker line corresponds to larger VAF. Colors of specific genomic alterations shown on the bottom.