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. 2022 Feb 15;23(4):283. doi: 10.3892/etm.2022.11213

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Overexpression of miR-150-5p suppresses osteogenic differentiation of AS fibroblasts. (A) Transfection efficiency. (B) mRNA expression of Col I, OPN and Runx2 determined by reverse transcription-quantitative PCR. (C) Protein levels of Col I, OPN and Runx2 determined by western blotting. (D) ALP activity. (E) Alizarin red staining for mineralization in BMP-2 and TGF-β1-induced AS fibroblasts transfected with miR-150-5p mimics or miR-NC or untreated cells (magnification, x100). Data are presented as the mean ± SD. ^**P<0.01, ^***P<0.001 vs. control; ^#P<0.05, ^##P<0.01, ^###P<0.001 vs. miR-NC. miR, microRNA; AS, ankylosing spondylitis; BMP, bone morphogenetic protein; TGF, transforming growth factor; Col I, collagen type I; OPN, osteopontin; ALP, alkaline phosphatase; NC, negative control.