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. 2022 Feb 16;23(4):290. doi: 10.3892/etm.2022.11219

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Copyright: © Yang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of intracellular calcium levels on GSN expression in HPDE6-C7 cells treated with CAE and TG. (A) Changes in intracellular calcium levels based on fluorescence imaging (x200 magnification) and (B) semi-quantitative analysis. (C) Protein expression levels of GSN, as determined by western blotting and (D) semi-quantitative analysis. (E) Relative mRNA expression levels of GSN, as determined by reverse transcription-quantitative polymerase chain reaction. The experiments were repeated at least three times. Data are presented as the mean ± standard deviation. ^**P<0.01. BC, blank control; CAE, caerulein; GSN, gelsolin; KD, knockdown; NC, negative control; TG, triglycerides.