Figure 3.

Effect of gelsolin on the major components of cell-cell junctions in HPDE6-C7 cells treated with CAE and TG. Relative mRNA expression levels of (A) E-cadherin, (B) nectin-2, (C) ZO-1 and (D) occludin, as determined by reverse transcription-quantitative polymerase chain reaction. (E) Western blot analysis and semi-quantification of the protein expression levels of (F) E-cadherin, (G) nectin-2, (H) ZO-1 and (I) occludin. The experiments were repeated at least three times. Data are presented as the mean ± standard deviation. ^*P<0.05, ^**P<0.01. BC, blank control; CAE, caerulein; KD, knockdown; NC, negative control; TG, triglycerides.