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Target gene primers used in reverse transcription-quantitative polymerase chain reaction.
| Gene | Forward primer (5'-3') | Reverse primer (5'-3') |
|---|---|---|
| GAPDH | ACATCGCTCAGACACCA | GTAGTTGAGGTCAATGAAGGG |
| GSN | AGCTGGCCAAGCTCTACAAG | TGTTTGCCTGCTTGCCTTTC |
| E-cadherin | AGGATGACACCCGGGACAAC | TGCAGCTGGCTCAAGTCAAAG |
| Nectin-2 | ACCTGCAAAGTGGAGCATGAGA | CCGGAGATGGACACTTCAGGA |
| ZO-1 | ATAAAGTGCTGGCTTGGTCTGTTTG | GCACTGCCCACCCATCTGTA |
| Occludin | AGTGCCACTTTGGCATTATGAGA | CTTGTGGCAGCAATTGGAAAC |
GSN, gelsolin.
