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. Author manuscript; available in PMC: 2023 Jan 18.
Published in final edited form as: Environ Sci Technol. 2022 Jan 5;56(2):1162–1173. doi: 10.1021/acs.est.1c06990

Figure 3.

Figure 3.

Anti-fog sprays and fluorinated components induce pre-adipocyte differentiation. 3T3-L1 pre-adipocytes were differentiated while exposed to sprays and constituent chemicals and then assayed for triglyceride accumulation (marker of adipocyte differentiation) via Nile red neutral lipid droplet staining. Triglyceride accumulation is depicted for the anti-fog spray commercial products (A) and for the fluorinated component chemicals and commercial mixtures (B). Data presented as percent triglyceride accumulation per cell (normalized to DNA content) relative to the maximal intraassay response for the rosiglitazone positive control. Data presented as mean ± SEM from three independent experiments. Data for triglyceride accumulation per cell at doses that were deemed cytotoxic are not shown.