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Cellular uptake of 2.5 μM DOX and DXNO-GR in MCF7 and MDA-MB-231 cells measured by flow cytometry in the absence (a) or in the presence (b) of the MDR-1 inhibitor verapamil (50 μM). Data are expressed as mean fluorescence intensity (a.u.) ± SD of at least three independent experiments, carried out in triplicate. *p < 0.001 significantly different from DOX (Student’s t-test); #p < 0.001 significantly different from-verapamil (Student’s t-test).
