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. 2022 Mar 10;41:89. doi: 10.1186/s13046-022-02301-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Grade1 PDAC is closely surrounded by abundant CAFs. A Representative immunofluorescence images for αSMA (red), CK19 (green), and DAPI (blue) in Grade1 and Grade3 portions of human PDAC. Scale bars, (left) 100 µm and (right) 50 µm. B Representative immunohistochemistry images for αSMA in surgically resected PDAC tissue of indicated tumor grades. Scale bars, 200 µm. C Quantification of αSMA staining area as a percentage of the total area in PDAC tissue of indicated tumor grades (n = 15). Data are presented as the mean ± SD (***P < 0.001)