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. 2022 Mar 10;41:89. doi: 10.1186/s13046-022-02301-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Differentiated PDOs are dependent on autocrine Wnt and exogenous RSPO. A Representative phase contrast images of Grade1, Grade2, and Grade3 PDOs cultured in niche medium or medium lacking the indicated factors. Scale bars, 100 µm. B Proliferation rate of PDOs cultured in the absence of the indicated factors compared with growth in full niche medium. C Representative phase contrast images of Grade1, Grade2, and Grade3 PDOs treated with vehicle (DMSO) or Porcn-inhibitor (C59) in niche medium lacking Wnt3A. Scale bars, 200 µm. D Proliferation rate of PDOs treated with C59 compared with PDOs treated with DMSO (**P < 0.01; ***P < 0.001)