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. Author manuscript; available in PMC: 2022 Mar 10.
Published in final edited form as: Cell Rep. 2019 Jun 11;27(11):3254–3268.e8. doi: 10.1016/j.celrep.2019.05.051

Figure 5. Poorer Kb-SIINFEKL presentation by neurons infected with T. gondii expressing vacOVA compared to neurons infected with T. gondii expressing GRA6-OVA.

Figure 5.

(a) Timeline of antigen presentation assay with Tg-infected primary neuronal cultures. AraC was added to inhibit growth of glial cells. (b, c) Tropism and infection rate of the two Tomato+ parasites following co-staining with MAP2 (green) and GFAP (blue). Pictures represent maximum intensity projections. Out of 24 fields, no vacuole was detected in a glial cell. Scale bar 10 μm. (d) Kb–SIINFEKL presentation by primary neurons infected for 24 h with the indicated tachyzoites, assessed by absorbance measurements following incubation with SIINFEKL-specific LacZ-inducible B3Z CD8 T cell hybridomas. (b, c, d) Representative of 2 independent experiments. See also Figure S3.