. 2022 Feb 22;12(5):545. doi: 10.3390/ani12050545

Table 2.

Primer used for real-time quantitative fluorescence PCR analysis.

Target Gene	Primer	Primer Sequence (5′-3′)	Product Length	Accession No.
β-Actin	Forward	TCCCTGGAGAAGAGCTATGAA	113 bp	NM_205518.1
β-Actin	Reverse	CAGGACTCCATACCCAAGAAAG	113 bp	NM_205518.1
IL-10	Forward	CCAGGGACGATGAACTTAACA	251 bp	NM_001004414.2
IL-10	Reverse	GATGGCTTTGCTCCTCTTCT	251 bp	NM_001004414.2
IL-1β	Forward	CTTCACCCTCAGCTTTCACG	137 bp	XM_015297469.2
IL-1β	Reverse	CCCTCCCATCCTTACCTTCT	137 bp	XM_015297469.2
IL-6	Forward	TTCAGAGTGACCTACACAGGC	146 bp	XM_015281283.2
IL-6	Reverse	GATGCTTTATCATGCGCTGC	146 bp	XM_015281283.2
TNF-α	Forward	GACAGCCTATGCCAACAAGTA	244 bp	AY765397.1
TNF-α	Reverse	TCCACATCTTTCAGAGCATCAA	244 bp	AY765397.1