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. 2022 Feb 24;10:816209. doi: 10.3389/fbioe.2022.816209

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Copyright © 2022 Zheng, Li, Zhang, Xu and Luo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-212-5p targeted ELF3 and regulated the expressions of ELF3 in SMSCs. (A) The predicted binding sequences of miR-212-5p with ELF3 mRNA were shown. (B) HEK-293T cells were co-transfected with luciferase reporters containing ELF3 3′-UTR (wide type or mutant) and miR-212-5p mimics or negative control. After 48 h of incubation, the relative luciferase activity was measured. n = 3. Mean ± SD. **p < 0.01, Two-way ANOVA followed Turkey’s multiple comparisons test. (C) qRT-PCR was used to assay the expressions of miR-212-5p in SMSCs and miR-212-5p overexpressed SMSCs. (D–F), SMSCs were transfected with miR-212-5p mimics or negative control for 48 h qRT-PCR and Western blotting were used to analyze the mRNA and proteins expressions of ELF3. N = 3 for each group. Mean ± SD. **p < 0.01, ***p < 0.001, One-way ANOVA followed Dunn’s multiple comparisons test.