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. 2022 Feb 24;10:816209. doi: 10.3389/fbioe.2022.816209

FIGURE 4.

FIGURE 4

Exosomes derived from miR-212-5p overexpressed SMSCs (SMSC-212-5p-Exos) inhibited up-regulated ELF3 expression in chondrocytes induced by IL-1β. (A) qRT-PCR was utilized to analyze the relative expressions of miR-212-5p from SMSC-Exos or SMSC-212-5p-Exos. (B) Cell viability of chondrocytes after individual treatment with different concentrations of IL-1β for 48 h. (C) Cell viability of chondrocytes after co-treatment with IL-1β and SMSC-Exos, or SMSC-212-5p-Exos for 48 h. (D–F) IL-1β induced chondrocytes were treated with SMSC-Exos or SMSC-212-5p-Exos for 48 h qRT-PCR and western blotting were used to analyze the mRNA and proteins expressions of ELF3. n = 6 for each group in cell viability analysis, n = 3 for each group in qRT-PCR and western blotting tests. #p < 0.05, ##p < 0.01, ###p < 0.001 compared to NC. @p < 0.05, @@p < 0.01, @@@p < 0.001 compared to IL-1β. &p < 0.05, &&p < 0.01 compared to IL-1β+Exo. One-way ANOVA followed Dunn’s multiple comparisons test.