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. 2022 Feb 25;14(4):1941–1958. doi: 10.18632/aging.203914

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Copyright: © 2022 Yin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fusobacterium nucleatum–induced intestinal microbial dysbiosis is involved in the development of colorectal cancer liver metastasis. (A–D) The rarefaction curve (A), relative abundance curve (B), Shannon diversity index (C), and Chao1 diversity index (D) of intestinal bacteria in mice treated with F. nucleatum or PBS were assessed by 16S rRNA sequencing (n=5 per group). Data are presented as mean ± standard deviation (SD; unpaired Student’s t-test). (E, F) The cecum and livers of mice were dissociated and weighed. The relative cecum weight (cecum weight/body weight) (E) and the metastatic liver weight/cecum weight (MLW/CW) ratio (F) were measured. Data are presented as mean ± SD (n=15 per group). *P < 0.05, **P < 0.01, and ***P < 0.001; unpaired Student’s t-test.