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. 2022 Feb 27;14(5):1230. doi: 10.3390/cancers14051230

Figure 4.

Figure 4

Metformin decreases ETC complexes activity in FLVCR1a-silenced cells. Activities of the mitochondrial electron transport chain complexes I–IV in H23 cells untreated (NT) and treated with 2.5 mM metformin for 24 or 48 h. FLVCR1a-silenced H23 cells are compared with cells expressing a scramble shRNA. Results were expressed as nmol of NAD+/min/mg of mitochondrial protein for complex I (A), nmol of reduced cytochrome c/min/mg of mitochondrial protein for complexes II–III (B,C), and nmol of oxidized cytochrome c/min/mg of mitochondrial protein for complex IV (D). Data represent means ± SEM, n = 3. For statistical analyses, a two-way analysis of variance was performed, followed by the Bonferroni correction for multiple groups comparisons * p < 0.05, ** p < 0.01, *** p < 0.001.