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. 2022 Feb 25;14(5):1195. doi: 10.3390/cancers14051195

Figure 2.

Figure 2

Proprotein convertases (PCs) expression and activity inhibition during enrichment of colon CSCs. (a–d), real time PCR analysis of Furin (a), PACE4 (b), PC5 (c) and PC7 (d) in colon CSCs and their parental cells in the colon cancer cells SW480, SW620, HT-29, and CT-26. (e–h), Effect of the PCs inhibitor α1-PDX (PDX) expression on the indicated parental colon cancer cells and corresponding CSCs was assessed by evaluating cells’ ability to digest the fluorogenic peptide pERTKR-MCA. (i,j), Effect of α1-PDX on the expression of Furin, PC5, PACE4 and PC7 in HT-29 (i) and CT-26 (j) cells. Results shown in the bar graphs represent PCs activity after 30–90 min of incubation and evaluated as raw fluorescence intensity (RFI). Results are representative of three experiments and data are mean ± S.E.M performed in triplicate. * p < 0.05. NS: not significant.