Figure 4.
The effect of GM-CSF on the expression of PD-L1, induced by IFN-γ in primary human basophils. Basophils (0.1 × 106 cells/200 μL/96-well plate) isolated from PBMCs of healthy donors were cultured with either IL-3, GM-CSF (5 ng/mL), GM-CSF + IL-3 or GM-CSF + IFN-γ. Basophil phenotype was evaluated by flow cytometry after 24 h. Expression of FcεRI, CD69, CD107a, PD-L1, and PD-L2 on the basophils (% positive cells and median fluorescence intensities (MFI), mean ± SD; n = 3 independent donors from three independent experiments) was presented. ns, not significant, * p < 0.05, one-way ANOVA Friedman test with Dunn’s multiple comparisons post-test.