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. 2022 Feb 26;11(5):823. doi: 10.3390/cells11050823

Figure 5.

Figure 5

Knockdown of KDM5 significantly impaired the osteogenic differentiation capacity of male NCSCs. (A) Schematic view of the siRNA design. (B) qPCR analysis validating the knockdown of KDM5D. (C) Validation of the knockdown of KDM5D at the protein level via Western Blot. (C–E) Knockdown of KDM5D strongly inhibited the capability of male NCSCs to deposit calcium after 30 days of culture on collagen type I fibers compared to untreated and GFP-transduced cells. (D–F) Quantification of Alizarin Red S confirmed the significant inhibition of osteogenic differentiation in male NCSCs after knockdown of KDM5D. Mann–Whitney test, * p < 0.05 was considered significant.