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. 2022 Feb 26;14(5):1217. doi: 10.3390/cancers14051217

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Multicolour Flow cytometry analysis of CT tumour associated immune cells after treatment. Percentages of (A) CD8⁺ T cells relative to CD3⁺ cells, (B) GZB+ CD8+ TILS relative to total CD8+ TILS, (C) CD8+ T_EM cells relative to total CD8⁺ cells (D) CD8+ T_EM cells relative to total CD8+ cells MFI (E) CD4+ T_EM cells relative to total CD4⁺ cells (F) CD4⁺ T_EM cells relative to total CD4⁺ cells MFI across all treatment arms. Data are shown as individual values with the mean ± S.D. and are representative of n = 5–10 mice/ group. * p < 0.05; ** p < 0.01; *** p < 0.001 compared to TNR.