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. 2022 Mar 1;11(5):848. doi: 10.3390/cells11050848

Figure 2.

Figure 2

Effect of PCOS on abdominal (ABD) and gluteofemoral (GF) adipose tissue secretome and cellular content. (A) Conditioned medium was collected from 18 paired ABD and GF adipose tissue explants (13 control and 5 PCOS women) and the amount of IL-8 was measured with ELISA. The Mann–Whitney test was used. * p = 0.07 ABD control vs. ABD PCOS. ** p = 0.03 GF control vs. GF PCOS. (B) Freshly isolated stroma vascular fraction (SVF) cells from ABD (left) and GF (right) adipose tissues were analyzed by FACS. Preadipocytes (CD34+ CD105+ CD31dim) were quantified and reported as the percentage of total SVF cells. Data are from n = 10 control and 4 PCOS women. The Mann–Whitney test was used. * p ≤ 0.1. (C) Freshly isolated PBMCs from 11 control and 4 PCOS women were analyzed by FACS and the number of EPCs (CD34+ KDR+) was quantified. The Mann–Whitney test was used. * p ≤ 0.1.