Figure 5.

Effect of biglycan and IGF-I on (A) U-2OS and (B) Saos-2 cells proliferation. Cells were harvested and seeded (4000 cells/well for U-2OS and 20,000 cells/well for Saos-2) on 96-well plates and were allowed to rest overnight. The next day the medium was replaced with 0% FBS DMEM for 24 h. Biglycan treatment was added in 0% FBS medium for the next 48 h at 37 °C and 5% CO₂. The cells were then lysed, and their number was calculated using the CyQUANT fluorometric assay kit. Results represent the average of three separate experiments. Means ± S.E.M were plotted; statistical significance: * p ≤ 0.05 *** p ≤ 0.001, not statistically significant (NS) compared with the respective control samples.