Figure 5.
NRF2 (nuclear factor erythroid 2–related factor 2), HO1 (heme oxygenase 1), JUN/AP1 (Jun proto-oncogene, AP-1 transcription factor subunit) gene expression and IL8 (CXCL8, interleukin 8) modulation under H2O2, EGF, LY and PD treatments alone and combined in Nthy-ori 3-1 cells (a) vs. TPC-1 cells (b). Gene expression was analyzed by real-time qPCR. The histogram represented normalized data with GUSB gene in Nthy-ori 3-1, while NRF2 and HO1 expression data were normalized with β-Actin in TPC-1 cells. The results showed the average of three independent experiments. LY—LY294002; PD—PD98059; *—p < 0.05; **—p < 0.01; ***—p < 0.001 treated vs. quiescent cells; #—p < 0.05, ##—p < 0.01, ###—p < 0.001 cells with similar treatment.