Figure 9.

Protein expression and quantification in Nthy-ori 3-1 vs. TPC-1 cells. Nthy-ori 3-1 and TPC-1 cells were starved overnight and treated with, LY294002 (25 μM), PD98059 (50μM), EGF (50 ng/mL) alone and combined with H₂O₂ (10 mM). Western blotting analysis in Nthy-ori 3-1 cells (a) and in TPC-1 cells (b) determining the protein expression levels of p-EGFR, ErbB2, p-MAPK, p-AKT and OGG1. Results are representative of three independent experiments. Quiescent cells were treated with H₂O₂ and LY, PD, EGF alone or combined. LY—LY294002; PD—PD98059; kDa—protein molecular weight marker.