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. 2022 Feb 28;11(5):831. doi: 10.3390/cells11050831

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of the SOOME in PCI3D modules on cell survival and growth. (A) A schematic PCI3D system design and its printed module image (right) reciprocally printed with PCL beams and cell-laden hydrogel beams. (B) Live/dead cell assay to detect the cells’ survival and proliferation at predetermined time points (1, 4 and 7 days after in vitro culture). Cells displayed calcein-AM positive (green) for live cells or EthD-1 negative (red) for dead cells. Scale bar = 100 µm. (C) The rate of live/dead cells and (D) proliferation activity was analyzed by imaging and CCK8 assay kit. The * symbol indicates p < 0.05 versus DMSO control by Two-way ANOVA; n = 3.