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. 2022 Feb 28;11(5):831. doi: 10.3390/cells11050831

Figure 7.

Figure 7

Effects of conditioned medium of SOOME on osteoblast differentiation and angiogenesis. The conditioned medium was collected from the supernatant of the medium of 24 h culture of SOOME and control CM. (A,B) Effect of conditioned medium on osteoblast differentiation of ST2. Osteoblast differentiation assays for (A) AP staining and (B) qPCR detection of the expression of osteoblast marker genes with or without conditioned medium. (C–F) Effects of conditioned medium on angiogenesis. Endothelial cell migration assay: imaging (C) and quantification (D) of migrated cells cultured in the transwell chamber with conditioned medium for 48 h; Imaging (E) and quantification of and node formation (F) of HUVEC cells cultured on Matrigel with conditioned medium. The * symbol indicates p < 0.05 versus control conditioned medium by t-test; n = 3. Scale bar = 100 µm. Green = branches; orange = master segments; blue sky = meshes; red surrounded by blue = nodes; Ct, cycle number detected by qPCR machine for a relative amount of target genes.