Figure 1.

Genomic analysis of X. tropicalis rho crispants. (A) Schematic of the Xenopus tropicalis rho gene. The depicted sequence corresponds to the CRISPR RNA targeting exon 1 and to the protospacer adjacent motif (PAM; indicated in bold). (B) Sequences obtained from individual clones of PCR products amplified from genomic DNA. Two mixtures (Mix1, 2) of 5 X. tropicalis F0 embryos injected with 500 pg of rho sgRNA and 600 pg of cas9 mRNA were used. For each clone, the targeted sequence is shown in pink, with PAM in bold, while indels are in blue. The right column indicates the corresponding protein sequence and its size (number of amino acids).