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. 2022 Mar 2;11(5):865. doi: 10.3390/cells11050865

Figure 3.

Figure 3

Lamin recruitment to nuclear ruptures is dependent on the lamin Ig-fold domain and BAF. NIH3T3 mouse fibroblast cells stably co-expressing cGAS-mCherry and GFP-tagged human (A) full-length LaA, (B) LaA rod (aa 1–435), or (C) LaA tail (aa 391–646) were depleted of endogenous A-type lamins by siRNA prior to laser-induced nuclear rupture. GFP-fusion protein accumulation at the rupture site was monitored for 10 min. Purple arrowheads indicate initial site of laser application. Yellow arrowheads indicate location of nuclear rupture. (D) BJ-5ta cells stably overexpressing GFP-LaA L530P and cGAS-mCherry were subjected to laser-induced nuclear rupture. (E) Representative images of BJ-5ta cells stably expressing GFP-LaA, GFP-LaC, GFP-LaA tail, or FTI-277-treated GFP-LaB1 that underwent laser-induced nuclear rupture following siRNA control or BAF knockdown. Scale bars, 10 μm.