Figure 3.

Combined treatment of CaMKII and NK1R inhibitors potently suppressed in vivo tumor growth of GSCs in a CAM model. Fertilized chick eggs were incubated in a humidified incubator at 37 °C. At embryonic day five, U87MG-derived GSCs were mixed with Matrigel in the absence or presence of the indicated compounds (5 μg/egg) and were implanted onto the CAM surface inside the silicone ring. Seven days later, the CAMs were photographed, the formed tumors were retrieved, and the tumor weight was calculated. ** p < 0.01, *** p < 0.001 vs. the compound alone.