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(A) Outer membrane preparations of defined and spontaneous PAO1 derived mutants. Lane 1, PAO1; lane 2, PASE1; lane 3, PA1433; lane 4, PA1436; lane 5, PA1434; lane 6, PA1423. Arrows indicate reduced expression of 55-kDa protein (lanes 3, 4) and presence of OprM (lane 5) and OprD (lane 6). (B) Western blots of total cell lysates obtained from the same strains as in panel A. The blots were revealed with anti-OprM antibody by using a chemiluminescent detection kit.
