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. 2022 Feb 23;11(5):783. doi: 10.3390/cells11050783

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Proportion and PD-L1 expression levels of circulating and intertumoral neutrophil subpopulations in LLC vs. LLC-GCSF models. NDN and LDF were isolated from the circulation of LLC and LLC-GCSF tumor-bearing mice and the tumors were harvested and digested. (A,B) The proportion of neutrophils in LDF (A) and whole tumor (B) was quantified based on Ly6G+ cells by flow cytometry. (C,E) The expression of PD-L1 was assessed in LDN (C), TAN (D) and NDN (E) by flow cytometry. Means values ± SE are presented, n = 7–30. Statistical significance was determined by unpaired two-tailed t-test with p < 0.05. **** p < 0.0001.