Figure 4.

The impact of modulating the NDN/LDN ratio on tumor growth. Mice bearing Lung K-Ras modified (LKRM) tumors were injected with 2–5 × 10⁶ NDN or 5 × 10⁶ LDN. Tumor growth was monitored along the treatments. Days of injections are marked by arrows. (A) Tumor growth ratio was calculated based on tumor size on each day vs. size day 0 and was assessed following different treatments over 3 days (Left panel). Tumor growth ratio at day 3 for each mouse is presented (Right panel). (B) Individual growth rate (mm³/day) was calculated for each mouse. (C) The proportion of TAN out of whole tumor following NDN or LDN adoptive transfer was assessed by flow cytometry. Mean values ± SE are shown, n = 6–8 in each group per experiment. Statistical significance was determined by one-way ANOVA with sidak’s post hoc test ((A)—Left panel), and by unpaired one/two-tailed t-test ((A)—Right panel, (B,C)), both with p < 0.05. * p < 0.05, ** p < 0.01, *** p < 0.001 and n.s—non-significant.