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. 2022 Mar 3;11(5):747. doi: 10.3390/foods11050747

Table 4.

Overview of HHP conditions applied for the inactivation of Alicyclobacillus species.

Alicyclobacillus Species Medium Experimental Conditions Reference
A. acidoterrestris ATCC49025
A. acidoterrestris NFPA 1013 (apple juice isolate)		 Apple juice 207, 414 and 621 MPa/1, 5 and 10 min/22, 45, 71 and 90 °C [264]
A. acidoterrestris DSMZ 2492 BAM broth
Orange juice
Tomato juice
Apple juice		 Broth
350 and 450 MPa/5, 10 and 20 min/35, 45 and 50 °C
Juices
350 MPa/20 min/50 °C and storage 3 weeks/30 °C		 [265]
A. acidoterrestris DSMZ 2492 BAM broth 350 and 450 MPa/35, 45 and 50 °C [266]
A. acidoterrestris NFPA 1101 (apple juice isolate)
A. acidoterrestris NFPA 1013 (apple juice isolate)		 Apple Juice concentrate 207, 414 and 621 MPa/1, 5 and 10 min/22, 45, 71 and 90 °C
Various concentrations of juice (17.5, 35 and 70 °Brix)		 [267]
A. acidoterrestris LMG 16906 Citric acid buffer (pH 4.0 and 5.0)
Potassium phosphate buffer (pH 7.0)
Tomato sauce (pH 4.2 and 5.0)		 HHP and combined treatment HHP + heat
Buffers
100, 200, 300, 400, 500 and 600 MPa/40 °C/10 min + heat 80 °C/10 min
Tomato sauce
100, 200, 300, 400, 500 and 600 MPa/25, 40 and 60 °C/10 min + heat 80 °C/10 min		 [268]
A. acidoterrestris TO-29/4/02 (apple juice isolate) Apple juice

  • 200, 300 and 500 MPa/30 min/50 °C continuously

  • 100, 200, 300 and 500 MPa/2, 4 and 6 cycles of 5 min with 5 min pause/50 °C

  • 100, 200 MPa × 6 cycles and 200 MPa x 4 cycles/5 min with 5 min pause/50 °C incubation 60 min at 50 °C/pressure 500 MPa/30 min 50 °C

  • Combined treatment of HHP + lysozyme: 300 MPa/5, 10, 15 and 30 min/50 °C + 0.05 and 0.1 mg/mL lysozyme

  • Combined treatment of HHP +nisin:
    • ◦
      300 MPa/5, 10, 15 and 30 min/50 °C + 500, 750 and 1000 IU/mL nisin
    • ◦
      200 MPa/5, 10, 15 and 20 min/50 °C + 250 IU/mL nisin
 [237]
A. acidoterrestris NZRM 4098 Orange juice 200 and 600 MPa/1–15 min/45, 55 and 65 °C [263]
A. acidoterrestris DSMZ 2498 Apple juice
Orange juice		 200, 400 and 600 MPa/10 min/20, 50 and 60 °C and storage for 28 days [98]
A. acidoterrestris TO-29/4/02 (apple juice isolate)
A. acidoterrestris TO-117/02 (apple juice isolate)		 Apple juice
(11.2, 23.6, 35.7 and 71.1 °Brix)		 200 MPa/5, 10, 15, 30 and 45 min/50 °C
200 MPa/5, 10, 15 and 30 min/50 °C for 11 days/11 and 16 months spores of TO-29/4/02
200 MPa/5, 10, 15 and 30 min/50 °C for 10 days/2, 10, 11 and 23 months spores of 117/02
200 MPa/1, 5, 10, 15 and 30 min/50 °C × 3 subsequent treatments		 [269]
A. acidoterrestris TO-117/02 (apple juice isolate) Mcllvain buffer
(pH 4.0 and 7.0)
Apple juice		 Germination and Inactivation

  • 100, 200, 300, 400 and 500 MPa/20 min/50 °C (buffers and juice)

  • 200 MPa/5, 10, 15 and 30 min/20, 50 and 70 °C (juice)

  • 200 MPa/5, 10, 15 and 30 min/11.3, 23.7, 35.5 and 70.7 °Brix

  • 200 and 500 MPa/20 min/50 and 70 °C (juice)

  • 200 MPa/5, 10, 15 and 30 min/50 °C (buffers and juice)

  • 200 MPa/50 °C for 2, 4 and 6 cycles/5 min with 5 min pause

 [270]
A. acidoterrestris TO-117/02 (apple juice isolate) Apple juice
Buffer (pH 4.0)		 200, 300, 400 and 500 MPa/15 min/4, 20 and 50 °C
Determination with optical density 		[271]
A. acidoterrestris NZRM 4447 Orange juice 200 and 600 MPa/15 min/39 °C + thermosonication 20.2 W/mL/78 °C [127]
A. acidoterrestris NZRM 4447 Malt Extract Broth
(10, 20 and 30 °Brix)		 600 MPa/up to 45 min/35, 45, 55 and 65 °C
600 MPa/up to 45 min/45 °C Validation for apple juice, lime juice concentrate and Blackcurrant juice concentrate		 [97]
A. acidoterrestris CCT 7547 Acai pulp 600 MPa/5, 10, 15, 20 and 25 min/65 °C [272]
A. acidoterrestris CCT 7547 Deionized Water 300 and 600 MPa/5 min/25 and 70 °C + heat shock [273]
A. acidoterrestris AJA 66 (apple juice isolate)
A. acidoterrestris ATCC 49025		 Apple juice
Potassium Phosphate Buffer (pH 3.7 and 7.0)		 600 MPa/1, 3 and 5 min/70, 80 and 90 °C [274]
A. acidoterrestris (apple juice isolate)
A. acidoterrestris DSMZ 2498		 Orange juice 500 and 600 MPa/1, 3, 5, 15 and 30 min/25, 45, 60 and 70 °C [275]
A. acidoterrestris CCT 7547 Mango pulp 600 MPa

  • 0, 5, 10, 15, 20 and 25 min/65 °C

  • 0, 4, 8, 12, 16 and 20 min/70 °C

  • 0, 2, 4, 6, 8 and 10 min/75 °C

  • 0, 1.5, 3, 4.5, 6 and 7.5 min/80 °C

  • 0, 1, 2, 3, 4 and 5 min/90 °C

 [276]
Alicyclobacillus spp. N1089 (canned tomatoes isolate)
Alicyclobacillus spp. N1098 (apple juice isolate)		 Tomato juice
Apple juice		 Combined treatment of HHP + Sucrose laurate L1695
392 MPa/10 min/45 °C + 0.005% and 0.01% for N1089
392 MPa/10 min/45 °C + 0.025, 0.04 and 0045% for N1098		 [247]