Figure 2.

Myeloid cell Dnmt3b deficiency does not affect the acute host response during Pseudomonas aeruginosa pneumonia. Myeloid cell-specific Dnmt3b-deficient mice (Dnmt3b^fl/flLysM^Cre) and littermate control (Dnmt3b^fl/fl) mice were infected with viable PAK (5 × 10⁶ CFU) via the airways and euthanized 6 or 24 h after infection. (A) Bacterial counts (colony forming units, CFU), (B) neutrophil numbers, (C) neutrophil CD11b expression, (D) myeloperoxidase (MPO), (E) elastase, (F) IL-β, IL-6 and TNF levels, all in bronchoalveolar lavage fluid (BALF). Data are shown as bar graphs with mean + SEM, n = 8. Differences between groups were not significant.