Table 1.
List of primary antibodies. Antibodies were diluted in TTBS supplemented with 0.4% BSA at the indicated dilutions.
| Antibody | Commercial Brand | Reference | Dilution |
|---|---|---|---|
| Rabbit mAb phospho-AKT Ser473 | Cell Signaling Technologies | 4058 | 1:2000 |
| Rabbit mAb phospho-AKT Thr308 | Cell Signaling Technologies | 4056 | 1:1000 |
| Rabbit mAb AKT | Cell Signaling Technologies | 4691 | 1:20,000 |
| Rabbit mAb phospho-IRβ | Cell Signaling Technologies | 3024 | 1:1000 |
| Rabbit mAb IRβ | Cell Signaling Technologies | 3025 | 1:2000 |
| Rabbit mAb phospho-ERK1/2 | Cell Signaling Technologies | 4370 | 1:4000 |
| Rabbit pAb ERK1/2 | Cell Signaling Technologies | 9102 | 1:4000 |
| Mouse mAb MET | Santa Cruz Biotechnologies | sc-8057 | 1:1000 |
| Mouse mAb MET | Cell Signaling Technologies | 3127 | 1:1000 |
| Mouse mAb α-Tubulin | Sigma-Aldrich/Merck | T5168 | 1:5000 |
| Mouse mAb Vinculin | Santa Cruz Biotechnologies | sc-73614 | 1:7500 |