Table 1.
Primer sequences for RT-qPCR gene expression analysis.
| Target | GenBank Accession Number |
Forward Reverse |
Sequence (5′→3′) |
|---|---|---|---|
| iNOS | NM_ 000625 | Forward Reverse |
ACCCAGACTTACCCCTTTGG GCCTGGGGTCTAGGAGAGAC |
| IL1β | NM_000576 | Forward Reverse |
GGGCCTCAAGGAAAAGAATC TTCTGCTTGAGAGGTGCTGA |
| IL6 | NM_000600 | Forward Reverse |
TACCCCCAGGAGAAGATTCC TTTTCTGCCAGTGCCTCTTT |
| TNFα | NM_000594 | Forward Reverse |
TCCTTCAGACACCCTCAACC AGGCCCCAGTTTGAATTCTT |
| IL10 | NM_000572 | Forward Reverse |
GCCTAACATGCTTCGAGATC TGATGTCTGGGTCTTGGTTC |
| CCR7 | NM_007719.2 | Forward Reverse |
GTGTGCTTCTGCCAAGATGA CCACGAAGCAGATGACAGAA |
| MRC1 | NM_ 138806 | Forward Reverse |
GGCGGTGACCTCACAAGTAT ACGAAGCCATTTGGTAAACG |
| HPRT | NM_001289746 | Forward Reverse |
ACCCCACGAAGTGTTGGATA AAGCAGATGGCCACAGAACT |
| GAPDH | NM_001289746 | Forward Reverse |
CACATGGCCTCCAAGGAGTAAG CCAGCAGTGAGGGTCTCTCT |