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. Author manuscript; available in PMC: 2022 Mar 10.
Published in final edited form as: Nat Metab. 2021 Oct 25;3(11):1552–1568. doi: 10.1038/s42255-021-00481-w

Extended Data Fig. 9 |. Adipose Ndufv2-mediated mitochondrial regulation is not a consequence of body weight.

Extended Data Fig. 9 |

Related to Fig. 7. Eight-week old female C57BL/6 J mice were fed a HF/HS diet for the first six weeks without any intervention, after which were injected with AAV vectors expressing either GFP or NDUFV2 in an adipose-specific manner and diet continued for six additional weeks. Metabolic traits such as (a) total mass; (b) fat and lean mass were monitored over 12 weeks. Comparisons of (c) tissue weights; (d) adipose Ndufv2 expression; (e) mitochondrial RCR; (f) coupling efficiency and (g) coupling respiration rates between GFP and NDUFV2 animals, respectively. Data are presented as mean ± SEM (n = 6 per group). P values were calculated using (A) Repeated measures 2-factor or (B) 3-factor or (C and G) 2-factor ANOVA corrected by post-hoc ‘Holm-Sidak’s’ multiple comparisons test; (D – F) Unpaired two-tailed Student’s t test.