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. 2022 Feb 23;23(5):2468. doi: 10.3390/ijms23052468

Figure 1.

Figure 1

Preparation of protein-drug/dye conjugates. (A) Schematic figure of enzymatic conversion with the formylglycine-generating enzyme (FGE) followed by tandem Knoevenagel ligation and strain-promoted azide-alkyne cycloaddition (SPAAC). (B) Schemes of the conjugated proteins DARPin monomer (DM), DARPin dimer with C-terminal CTPSR tag (DD1), DARPinFc (DFc) with the dimerized EGFR ectodomain (PDB: 3NJP) in the background and scFv425Fc (scFvFc). Molecular weight is given for the native protein. Composite models based on DARPin (PDB: 3NOC), Fab (PDB: 2IG2), Fc (PDB: 1FC2) and hinge as described in Padlan, 1994 [47], visualization with UCSF Chimera.