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PCR conditions for mice genotyping.
| Temperature | Time | Cycles | |
|---|---|---|---|
| APP PCR | |||
| Denaturation | 93 °C | 3 min | 1 |
| Denaturation | 93 °C | 15 s | 40 |
| Annealing | 56 °C | 30 s | |
| Extension | 68 °C | 1 min | 1 |
| BIN/COPS5 PCR | |||
| Denaturation | 98 °C | 30 s | 1 |
| Denaturation | 98 °C | 5 s | 40 |
| Annealing | 52 °C | 5 s | |
| Extension | 72 °C | 15 s | 1 |
| Extension | 72 °C | 1 min | 1 |
